Hotspot

psbZ-trnfM-CAU

Genome · 106984-107762 · 779 bp

Back to Cicer

Pi 0.0323
MarkerSeek score 74.30
Primer available yes
Label psbZ-trnfM-CAU

Feature Metadata

label_name
psbZ-trnfM-CAU
feature_type
igs
region
Genome
length_bp
779
variable_sites
40
pi
0.0323
unique_haplotype_count
4
primer_available
yes
alignment_reliability
0.93
markerseek_score
74.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
psbZ-trnfM-CAU_p1 CCGGTACATCATTATGGATTGGA CTTGAGGTCACGGGTTCAAA 43.5 50.0 58.1 58.0 831-882 bp 1.0 62.7
psbZ-trnfM-CAU_p2 TCCGGTACATCATTATGGATTGG CTTGAGGTCACGGGTTCAAA 43.5 50.0 58.1 58.0 832-883 bp 1.0 62.7
psbZ-trnfM-CAU_p3 CCGGTACATCATTATGGATTGGA TGAGGTCACGGGTTCAAATC 43.5 50.0 58.1 57.8 829-880 bp 1.0 61.6
psbZ-trnfM-CAU_p4 CCGGTACATCATTATGGATTGGA TTTTTCGCGGAGTAGAGCAA 43.5 45.0 58.1 57.8 876-927 bp 1.0 61.5
psbZ-trnfM-CAU_p5 TCCGGTACATCATTATGGATTGG TTTTTCGCGGAGTAGAGCAA 43.5 45.0 58.1 57.8 877-928 bp 1.0 61.5