Hotspot

trnY-GUA-trnE-UUC

Genome · 101318-101377 · 60 bp

Back to Cicer

Pi 0.0722
MarkerSeek score 75.90
Primer available yes
Label trnY-GUA-trnE-UUC

Feature Metadata

label_name
trnY-GUA-trnE-UUC
feature_type
igs
region
Genome
length_bp
60
variable_sites
7
pi
0.0722
unique_haplotype_count
4
primer_available
yes
alignment_reliability
1.00
markerseek_score
75.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnY-GUA-trnE-UUC_p1 CGAATTTACAGTCCGTCCCC TCTCTTTCAAGGAGGCAACG 55.0 50.0 58.4 57.8 147 bp 1.0 79.6
trnY-GUA-trnE-UUC_p2 ACGAATTTACAGTCCGTCCC TCTCTTTCAAGGAGGCAACG 50.0 50.0 57.6 57.8 148 bp 1.0 79.4
trnY-GUA-trnE-UUC_p3 GAATTTACAGTCCGTCCCCA TCTCTTTCAAGGAGGCAACG 50.0 50.0 57.2 57.8 146 bp 1.0 76.3
trnY-GUA-trnE-UUC_p4 CGAATTTACAGTCCGTCCCC CTCTTTCAAGGAGGCAACGG 55.0 55.0 58.4 58.8 146 bp 1.0 74.3
trnY-GUA-trnE-UUC_p5 ACGAATTTACAGTCCGTCCC CTCTTTCAAGGAGGCAACGG 50.0 55.0 57.6 58.8 147 bp 1.0 74.1