Hotspot

atpF-atpH

Genome · 83057-83576 · 520 bp

Back to Cicer

Pi 0.0344
MarkerSeek score 73.80
Primer available yes
Label atpF-atpH

Feature Metadata

label_name
atpF-atpH
feature_type
igs
region
Genome
length_bp
520
variable_sites
29
pi
0.0344
unique_haplotype_count
4
primer_available
yes
alignment_reliability
0.97
markerseek_score
73.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
atpF-atpH_p1 GATGGCCAGTAACTCAAGCA TGGGCTGGTTGTAGCATTAG 50.0 50.0 57.9 57.6 598-616 bp 0.75 72.2
atpF-atpH_p2 CGGATGGCCAGTAACTCAAG TGGGCTGGTTGTAGCATTAG 55.0 50.0 58.3 57.6 600-618 bp 0.75 70.3
atpF-atpH_p3 TGGCCAGTAACTCAAGCAAA TGGGCTGGTTGTAGCATTAG 45.0 50.0 57.6 57.6 596-614 bp 0.75 70.3
atpF-atpH_p4 GATGGCCAGTAACTCAAGCA TTTATGGGCTGGTTGTAGCA 50.0 45.0 57.9 57.1 602-620 bp 0.75 68.4
atpF-atpH_p5 ATGGCCAGTAACTCAAGCAA TGGGCTGGTTGTAGCATTAG 45.0 50.0 57.4 57.6 597-615 bp 0.75 68.4