Hotspot

accD-trnQ-UUG

Genome · 75223-76565 · 1343 bp

Back to Cicer

Pi 0.0386
MarkerSeek score 73.10
Primer available yes
Label accD-trnQ-UUG

Feature Metadata

label_name
accD-trnQ-UUG
feature_type
igs
region
Genome
length_bp
1343
variable_sites
85
pi
0.0386
unique_haplotype_count
4
primer_available
yes
alignment_reliability
0.98
markerseek_score
73.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
accD-trnQ-UUG_p1 AGGGTTCCAAGTACCTGGAT GAGGTTCGAATCCTTCCGTC 50.0 55.0 57.6 58.1 2090-2136 bp 1.0 83.6
accD-trnQ-UUG_p2 ACTTTCGCATTCAACCCACA GAGGTTCGAATCCTTCCGTC 45.0 55.0 58.3 58.1 1982-2007 bp 0.75 73.0
accD-trnQ-UUG_p3 ACTTTCGCATTCAACCCACA TCAGACCCTCAACTCCCTTT 45.0 50.0 58.3 57.9 2279-2304 bp 0.75 72.5
accD-trnQ-UUG_p4 ACTTTCGCATTCAACCCACA AGATCAGACCCTCAACTCCC 45.0 55.0 58.3 58.1 2282-2307 bp 0.75 72.4
accD-trnQ-UUG_p5 ACTTTCGCATTCAACCCACA GATCAGACCCTCAACTCCCT 45.0 55.0 58.3 58.1 2281-2306 bp 0.75 72.4