Hotspot

rpl2-rps19

Genome · 48628-48830 · 203 bp

Back to Cicer

Pi 0.0771
MarkerSeek score 78.00
Primer available yes
Label rpl2-rps19

Feature Metadata

label_name
rpl2-rps19
feature_type
igs
region
Genome
length_bp
203
variable_sites
29
pi
0.0771
unique_haplotype_count
4
primer_available
yes
alignment_reliability
0.92
markerseek_score
78.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
rpl2-rps19_p1 GATTCTTCGTCGCCGTACTA TGGTAGATGCTCTGGACCAA 50.0 50.0 57.3 58.0 325-346 bp 1.0 84.6
rpl2-rps19_p2 GATTCTTCGTCGCCGTACTA ATGGTAGATGCTCTGGACCA 50.0 50.0 57.3 57.8 326-347 bp 1.0 83.5
rpl2-rps19_p3 TTCTTCGTCGCCGTACTAAA TGGTAGATGCTCTGGACCAA 45.0 50.0 57.0 58.0 323-344 bp 1.0 82.7
rpl2-rps19_p4 GATTCTTCGTCGCCGTACTAA TGGTAGATGCTCTGGACCAA 47.6 50.0 57.9 58.0 325-346 bp 1.0 82.1
rpl2-rps19_p5 TTCTTCGTCGCCGTACTAAA ATGGTAGATGCTCTGGACCA 45.0 50.0 57.0 57.8 324-345 bp 1.0 81.6