Hotspot

trnI-GAU-rrn16

Genome · 32375-32672 · 298 bp

Back to Cicer

Pi 0.036
MarkerSeek score 74.70
Primer available yes
Label trnI-GAU-rrn16

Feature Metadata

label_name
trnI-GAU-rrn16
feature_type
igs
region
Genome
length_bp
298
variable_sites
19
pi
0.036
unique_haplotype_count
4
primer_available
yes
alignment_reliability
0.99
markerseek_score
74.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnI-GAU-rrn16_p1 GGCACAACGACGCAATTATC CAAGGTAGCCGTACTGGAAG 50.0 55.0 58.2 57.7 390-392 bp 1.0 80.4
trnI-GAU-rrn16_p2 GGCACAACGACGCAATTATC AGTGACTGGAGTGAAGTCGT 50.0 50.0 58.2 58.3 412-414 bp 1.0 80.1
trnI-GAU-rrn16_p3 GGCACAACGACGCAATTATC AACAAGGTAGCCGTACTGGA 50.0 50.0 58.2 58.4 392-394 bp 1.0 79.9
trnI-GAU-rrn16_p4 GGCACAACGACGCAATTATC ACAAGGTAGCCGTACTGGAA 50.0 50.0 58.2 58.4 391-393 bp 1.0 79.9
trnI-GAU-rrn16_p5 GGCACAACGACGCAATTATC AAGTCGTAACAAGGTAGCCG 50.0 50.0 58.2 57.6 399-401 bp 1.0 79.8