Hotspot

psbA-trnH-GUG

Genome · 6001-6342 · 342 bp

Back to Cicer

Pi 0.05
MarkerSeek score 76.90
Primer available yes
Label psbA-trnH-GUG

Feature Metadata

label_name
psbA-trnH-GUG
feature_type
igs
region
Genome
length_bp
342
variable_sites
28
pi
0.05
unique_haplotype_count
4
primer_available
yes
alignment_reliability
0.99
markerseek_score
76.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
psbA-trnH-GUG_p1 GGTATCTGGTTTACCGCGTT CAATCCACTGCCTTGATCCA 50.0 50.0 58.0 57.9 581-600 bp 1.0 86.6
psbA-trnH-GUG_p2 GGTATCTGGTTTACCGCGTT AATCCACTGCCTTGATCCAC 50.0 50.0 58.0 57.9 580-599 bp 1.0 86.6
psbA-trnH-GUG_p3 GGTATCTGGTTTACCGCGTT ACAATCCACTGCCTTGATCC 50.0 50.0 58.0 57.9 582-601 bp 1.0 86.6
psbA-trnH-GUG_p4 GTTATACAATGGCGGTCCGT CAATCCACTGCCTTGATCCA 50.0 50.0 58.1 57.9 1110-1129 bp 1.0 82.8
psbA-trnH-GUG_p5 GTTATACAATGGCGGTCCGT AATCCACTGCCTTGATCCAC 50.0 50.0 58.1 57.9 1109-1128 bp 1.0 82.8