primer_id	label_name	rank	fwd_seq	rev_seq	fwd_len	rev_len	fwd_gc	rev_gc	fwd_tm	rev_tm	fwd_self_any_th	rev_self_any_th	primer3_penalty	target_start	target_end	amplicon_size	cross_species_success_rate	primer_score
rbcL-trnK-UUU_p1	rbcL-trnK-UUU	1	AGTCCTGAATTAGCTGCTGC	TGGAGTCGCACTTAAAAGCC	20	20	50.0	50.0	58.0	58.5	13.4	0.0	0.516122	1402	2065	783-818	1.000000	80.0
rbcL-trnK-UUU_p2	rbcL-trnK-UUU	2	AGTCCTGAATTAGCTGCTGC	AACCCTACCGATGGTATGGA	20	20	50.0	50.0	58.0	57.5	13.4	20.0	0.517944	1402	2065	836-871	1.000000	79.7
rbcL-trnK-UUU_p3	rbcL-trnK-UUU	3	AGTCCTGAATTAGCTGCTGC	AGTCGCACTTAAAAGCCGAG	20	20	50.0	50.0	58.0	58.6	13.4	6.4	0.606067	1402	2065	780-815	1.000000	79.3
rbcL-trnK-UUU_p4	rbcL-trnK-UUU	4	AGTCCTGAATTAGCTGCTGC	AGTCGTGGTCTTACAAACCC	20	20	50.0	50.0	58.0	57.5	13.4	0.0	0.574042	1402	2065	851-886	1.000000	79.2
rbcL-trnK-UUU_p5	rbcL-trnK-UUU	5	AGTCCTGAATTAGCTGCTGC	GTCGTGGTCTTACAAACCCT	20	20	50.0	50.0	58.0	57.5	13.4	0.0	0.574042	1402	2065	850-885	1.000000	79.2
psbA-trnH-GUG_p1	psbA-trnH-GUG	1	GGTATCTGGTTTACCGCGTT	CAATCCACTGCCTTGATCCA	20	20	50.0	50.0	58.0	57.9	4.9	0.0	0.147617	6001	6342	581-600	1.000000	86.6
psbA-trnH-GUG_p2	psbA-trnH-GUG	2	GGTATCTGGTTTACCGCGTT	AATCCACTGCCTTGATCCAC	20	20	50.0	50.0	58.0	57.9	4.9	0.0	0.149124	6001	6342	580-599	1.000000	86.6
psbA-trnH-GUG_p3	psbA-trnH-GUG	3	GGTATCTGGTTTACCGCGTT	ACAATCCACTGCCTTGATCC	20	20	50.0	50.0	58.0	57.9	4.9	0.0	0.149124	6001	6342	582-601	1.000000	86.6
psbA-trnH-GUG_p4	psbA-trnH-GUG	4	GTTATACAATGGCGGTCCGT	CAATCCACTGCCTTGATCCA	20	20	50.0	50.0	58.1	57.9	13.9	0.0	0.189250	6001	6342	1110-1129	1.000000	82.8
psbA-trnH-GUG_p5	psbA-trnH-GUG	5	GTTATACAATGGCGGTCCGT	AATCCACTGCCTTGATCCAC	20	20	50.0	50.0	58.1	57.9	13.9	0.0	0.190757	6001	6342	1109-1128	1.000000	82.8
ycf1_p1	ycf1	1	CTCGGGAGAAATCTGGTTCG	AACAAGTTCCTCGATGGTCA	20	20	55.0	45.0	58.1	56.8	0.0	0.0	1.293694	23464	23583	216-282	1.000000	81.7
ycf1_p2	ycf1	2	CTCGGGAGAAATCTGGTTCG	ACGTTTTCGAGGACAAATCCA	20	21	55.0	42.9	58.1	58.2	0.0	0.0	1.230890	23464	23583	602-668	1.000000	81.1
ycf1_p3	ycf1	3	TGTCTTCAGCCTCGTCTTTG	ACGTTTTCGAGGACAAATCCA	20	21	50.0	42.9	57.8	58.2	0.0	0.0	1.313639	23464	23583	860-926	1.000000	80.1
ycf1_p4	ycf1	4	TGTGCCAAGGTTTCAGAACA	ACGTTTTCGAGGACAAATCCA	20	21	45.0	42.9	57.9	58.2	0.0	0.0	1.295083	23464	23583	1175-1241	1.000000	79.2
ycf1_p5	ycf1	5	TGCTTTCCCAGGATTCTTCG	ACGTTTTCGAGGACAAATCCA	20	21	50.0	42.9	57.9	58.2	0.0	0.0	1.272645	23464	23583	953-953	0.250000	39.8
trnN-GUU-trnR-ACG_p1	trnN-GUU-trnR-ACG	1	CCGTTGTTCCTCAGTAGCTC	CAGAGGATTAGAGCACGTGG	20	20	55.0	55.0	58.0	57.8	0.0	18.9	0.218238	26005	26614	750-768	1.000000	85.4
trnN-GUU-trnR-ACG_p2	trnN-GUU-trnR-ACG	2	ATTTGCCGTTGTTCCTCAGT	CAGAGGATTAGAGCACGTGG	20	20	45.0	55.0	58.0	57.8	0.0	18.9	0.234651	26005	26614	755-773	1.000000	85.2
trnN-GUU-trnR-ACG_p3	trnN-GUU-trnR-ACG	3	TTGCCGTTGTTCCTCAGTAG	CAGAGGATTAGAGCACGTGG	20	20	50.0	55.0	57.8	57.8	0.0	18.9	0.379725	26005	26614	753-771	1.000000	84.0
trnN-GUU-trnR-ACG_p4	trnN-GUU-trnR-ACG	4	TTCCTCAGTAGCTCAGTGGT	CAGAGGATTAGAGCACGTGG	20	20	50.0	55.0	57.7	57.8	0.0	18.9	0.526686	26005	26614	744-762	1.000000	82.9
trnN-GUU-trnR-ACG_p5	trnN-GUU-trnR-ACG	5	CCGATTGGTCGTAGGTTCAA	CAGAGGATTAGAGCACGTGG	20	20	50.0	55.0	57.6	57.8	0.0	18.9	0.589447	26005	26614	706-724	1.000000	82.8
trnI-GAU-rrn16_p1	trnI-GAU-rrn16	1	GGCACAACGACGCAATTATC	CAAGGTAGCCGTACTGGAAG	20	20	50.0	55.0	58.2	57.7	0.0	7.8	0.485056	32375	32672	390-392	1.000000	80.4
trnI-GAU-rrn16_p2	trnI-GAU-rrn16	2	GGCACAACGACGCAATTATC	AGTGACTGGAGTGAAGTCGT	20	20	50.0	50.0	58.2	58.3	0.0	0.0	0.496177	32375	32672	412-414	1.000000	80.1
trnI-GAU-rrn16_p3	trnI-GAU-rrn16	3	GGCACAACGACGCAATTATC	AACAAGGTAGCCGTACTGGA	20	20	50.0	50.0	58.2	58.4	0.0	7.8	0.553655	32375	32672	392-394	1.000000	79.9
trnI-GAU-rrn16_p4	trnI-GAU-rrn16	4	GGCACAACGACGCAATTATC	ACAAGGTAGCCGTACTGGAA	20	20	50.0	50.0	58.2	58.4	0.0	7.8	0.553655	32375	32672	391-393	1.000000	79.9
trnI-GAU-rrn16_p5	trnI-GAU-rrn16	5	GGCACAACGACGCAATTATC	AAGTCGTAACAAGGTAGCCG	20	20	50.0	50.0	58.2	57.6	0.0	0.0	0.548742	32375	32672	399-401	1.000000	79.8
ycf2_p1	ycf2	1	CATGGTTCGATCCTGCAAGA	CCGACAGAAAGTCCGTTTGA	20	20	50.0	50.0	58.0	58.1	13.6	0.0	0.167744	40643	40762	363-375	0.750000	79.2
ycf2_p2	ycf2	2	CATGGTTCGATCCTGCAAGA	TTTTGAGTTGTTCTGCCGGA	20	20	50.0	45.0	58.0	58.0	13.6	5.0	0.073981	40643	40762	518-530	0.750000	78.7
ycf2_p3	ycf2	3	GGTTCGATCCTGCAAGAACT	TTTTGAGTTGTTCTGCCGGA	20	20	50.0	45.0	57.9	58.0	5.2	5.0	0.146166	40643	40762	515-527	0.750000	78.2
ycf2_p4	ycf2	4	GGCTTATTCGAAAAACGCCC	TTTTGAGTTGTTCTGCCGGA	20	20	50.0	45.0	58.1	58.0	16.2	5.0	0.141417	40643	40762	573-585	0.750000	77.8
ycf2_p5	ycf2	5	CCCGAAATGACCTTTCCCAA	TTTTGAGTTGTTCTGCCGGA	20	20	50.0	45.0	58.1	58.0	0.0	5.0	0.127689	40643	40762	456-456	0.500000	61.8
rpl2-rps19_p1	rpl2-rps19	1	GATTCTTCGTCGCCGTACTA	TGGTAGATGCTCTGGACCAA	20	20	50.0	50.0	57.3	58.0	0.0	0.0	0.793578	48628	48830	325-346	1.000000	84.6
rpl2-rps19_p2	rpl2-rps19	2	GATTCTTCGTCGCCGTACTA	ATGGTAGATGCTCTGGACCA	20	20	50.0	50.0	57.3	57.8	0.0	9.1	0.925733	48628	48830	326-347	1.000000	83.5
rpl2-rps19_p3	rpl2-rps19	3	TTCTTCGTCGCCGTACTAAA	TGGTAGATGCTCTGGACCAA	20	20	45.0	50.0	57.0	58.0	0.0	0.0	1.036204	48628	48830	323-344	1.000000	82.7
rpl2-rps19_p4	rpl2-rps19	4	GATTCTTCGTCGCCGTACTAA	TGGTAGATGCTCTGGACCAA	21	20	47.6	50.0	57.9	58.0	0.0	0.0	1.105742	48628	48830	325-346	1.000000	82.1
rpl2-rps19_p5	rpl2-rps19	5	TTCTTCGTCGCCGTACTAAA	ATGGTAGATGCTCTGGACCA	20	20	45.0	50.0	57.0	57.8	0.0	9.1	1.168359	48628	48830	324-345	1.000000	81.6
rpl20-rps18_p1	rpl20-rps18	1	TCTGTACAAGAAGCAATTGC	AGCGAGTCGATCACTAGAAC	20	20	40.0	50.0	54.2	56.9	23.1	18.6	4.899223	64301	64570	422-430	1.000000	45.9
rpl20-rps18_p2	rpl20-rps18	2	TCTGTACAAGAAGCAATTGC	GCGAGTCGATCACTAGAACT	20	20	40.0	50.0	54.2	56.9	23.1	18.6	4.899223	64301	64570	421-429	1.000000	45.9
rpl20-rps18_p3	rpl20-rps18	3	TCTGTACAAGAAGCAATTGC	AGCGAGTCGATCACTAGAACT	20	21	40.0	47.6	54.2	58.4	23.1	18.6	5.146234	64301	64570	422-430	1.000000	45.1
rpl20-rps18_p4	rpl20-rps18	4	TCTGTACAAGAAGCAATTGC	AAGCGAGTCGATCACTAGAAC	20	21	40.0	47.6	54.2	57.6	23.1	18.6	5.189122	64301	64570	423-431	1.000000	45.1
rpl20-rps18_p5	rpl20-rps18	5	ATCTGTACAAGAAGCAATTGC	GCGAGTCGATCACTAGAACT	21	20	38.1	50.0	54.8	56.9	23.1	18.6	5.280420	64301	64570	422-430	1.000000	45.1
accD-trnQ-UUG_p1	accD-trnQ-UUG	1	AGGGTTCCAAGTACCTGGAT	GAGGTTCGAATCCTTCCGTC	20	20	50.0	55.0	57.6	58.1	14.0	16.9	0.437186	75223	76565	2090-2136	1.000000	83.6
accD-trnQ-UUG_p2	accD-trnQ-UUG	2	ACTTTCGCATTCAACCCACA	GAGGTTCGAATCCTTCCGTC	20	20	45.0	55.0	58.3	58.1	0.0	16.9	0.389746	75223	76565	1982-2007	0.750000	73.0
accD-trnQ-UUG_p3	accD-trnQ-UUG	3	ACTTTCGCATTCAACCCACA	TCAGACCCTCAACTCCCTTT	20	20	45.0	50.0	58.3	57.9	0.0	0.0	0.432049	75223	76565	2279-2304	0.750000	72.5
accD-trnQ-UUG_p4	accD-trnQ-UUG	4	ACTTTCGCATTCAACCCACA	AGATCAGACCCTCAACTCCC	20	20	45.0	55.0	58.3	58.1	0.0	0.0	0.440873	75223	76565	2282-2307	0.750000	72.4
accD-trnQ-UUG_p5	accD-trnQ-UUG	5	ACTTTCGCATTCAACCCACA	GATCAGACCCTCAACTCCCT	20	20	45.0	55.0	58.3	58.1	0.0	0.0	0.440873	75223	76565	2281-2306	0.750000	72.4
trnQ-UUG-psbK_p1	trnQ-UUG-psbK	1	GACCAAAACCCGTTGCCTTA	ACTTGAATAAAGGGCAGAGTCA	20	22	50.0	40.9	58.7	57.4	0.0	0.0	3.320265	76638	77274	732-761	1.000000	58.7
trnQ-UUG-psbK_p2	trnQ-UUG-psbK	2	CGAATAACGGGACCAAAACC	ACTTGAATAAAGGGCAGAGTCA	20	22	50.0	40.9	57.1	57.4	0.0	0.0	3.546731	76638	77274	742-771	1.000000	56.8
trnQ-UUG-psbK_p3	trnQ-UUG-psbK	3	ACCAAAACCCGTTGCCTTAC	ACTTGAATAAAGGGCAGAGTCA	20	22	50.0	40.9	59.0	57.4	0.0	0.0	3.606415	76638	77274	731-760	1.000000	56.4
trnQ-UUG-psbK_p4	trnQ-UUG-psbK	4	GACCAAAACCCGTTGCCTTA	ACTTGAATAAAGGGCAGAGTCAA	20	23	50.0	39.1	58.7	58.0	0.0	0.0	3.693955	76638	77274	732-761	1.000000	55.7
trnQ-UUG-psbK_p5	trnQ-UUG-psbK	5	CGAATAACGGGACCAAAACC	ACTTGAATAAAGGGCAGAGTCAA	20	23	50.0	39.1	57.1	58.0	0.0	0.0	3.920421	76638	77274	742-771	1.000000	53.8
trnR-UCU-atpA_p1	trnR-UCU-atpA	1	TTCAAATCCTATTGGGCGCA	TTCACTGGGGAAGCAGAAAC	20	20	45.0	50.0	57.9	58.0	10.8	0.0	0.155042	80023	80213	301-310	1.000000	85.6
trnR-UCU-atpA_p2	trnR-UCU-atpA	2	TTCAAATCCTATTGGGCGCA	CATTCACTGGGGAAGCAGAA	20	20	45.0	50.0	57.9	57.8	10.8	0.0	0.341321	80023	80213	303-312	1.000000	84.0
trnR-UCU-atpA_p3	trnR-UCU-atpA	3	TTCAAATCCTATTGGGCGCA	AAGACATTCACTGGGGAAGC	20	20	45.0	50.0	57.9	57.8	10.8	0.0	0.342875	80023	80213	307-316	1.000000	83.9
trnR-UCU-atpA_p4	trnR-UCU-atpA	4	TTCAAATCCTATTGGGCGCA	GGGGAAGCAGAAACCCTTTT	20	20	45.0	50.0	57.9	58.3	10.8	0.0	0.419267	80023	80213	295-304	1.000000	83.6
trnR-UCU-atpA_p5	trnR-UCU-atpA	5	AAGAAAAGCGCCCATTGTCT	TTCACTGGGGAAGCAGAAAC	20	20	45.0	50.0	58.4	58.0	2.3	0.0	0.397921	80023	80213	360-369	1.000000	82.5
atpF-atpH_p1	atpF-atpH	1	GATGGCCAGTAACTCAAGCA	TGGGCTGGTTGTAGCATTAG	20	20	50.0	50.0	57.9	57.6	13.0	0.0	0.532228	83057	83576	598-616	0.750000	72.2
atpF-atpH_p2	atpF-atpH	2	CGGATGGCCAGTAACTCAAG	TGGGCTGGTTGTAGCATTAG	20	20	55.0	50.0	58.3	57.6	13.0	0.0	0.761716	83057	83576	600-618	0.750000	70.3
atpF-atpH_p3	atpF-atpH	3	TGGCCAGTAACTCAAGCAAA	TGGGCTGGTTGTAGCATTAG	20	20	45.0	50.0	57.6	57.6	0.0	0.0	0.775033	83057	83576	596-614	0.750000	70.3
atpF-atpH_p4	atpF-atpH	4	GATGGCCAGTAACTCAAGCA	TTTATGGGCTGGTTGTAGCA	20	20	50.0	45.0	57.9	57.1	13.0	0.0	1.003030	83057	83576	602-620	0.750000	68.4
atpF-atpH_p5	atpF-atpH	5	ATGGCCAGTAACTCAAGCAA	TGGGCTGGTTGTAGCATTAG	20	20	45.0	50.0	57.4	57.6	13.0	0.0	1.003203	83057	83576	597-615	0.750000	68.4
atpH-atpI_p1	atpH-atpI	1	GCAGCAATAACAGAAGCAGC	GTGAATCCATGGAAGGGCAT	20	20	50.0	50.0	58.1	57.9	0.0	20.7	0.172238	83823	85080	1222-1322	1.000000	86.4
atpH-atpI_p2	atpH-atpI	2	ATAGAAGCAAGTCCAACGGC	GTGAATCCATGGAAGGGCAT	20	20	50.0	50.0	58.3	57.9	0.0	20.7	0.338621	83823	85080	1249-1349	1.000000	85.0
atpH-atpI_p3	atpH-atpI	3	GCAGCAATAACAGAAGCAGC	GAATCCATGGAAGGGCATCA	20	20	50.0	50.0	58.1	57.6	0.0	20.7	0.464534	83823	85080	1220-1320	1.000000	84.1
atpH-atpI_p4	atpH-atpI	4	CTGTGCCTTGACCAATTCCA	GTGAATCCATGGAAGGGCAT	20	20	50.0	50.0	58.4	57.9	0.0	20.7	0.458033	83823	85080	1275-1375	1.000000	84.0
atpH-atpI_p5	atpH-atpI	5	TGTGCCTTGACCAATTCCAG	GTGAATCCATGGAAGGGCAT	20	20	50.0	50.0	58.4	57.9	0.0	20.7	0.458033	83823	85080	1274-1374	1.000000	84.0
trnY-GUA-trnE-UUC_p1	trnY-GUA-trnE-UUC	1	CGAATTTACAGTCCGTCCCC	TCTCTTTCAAGGAGGCAACG	20	20	55.0	50.0	58.4	57.8	0.0	0.0	0.521328	101318	101377	147-147	1.000000	79.6
trnY-GUA-trnE-UUC_p2	trnY-GUA-trnE-UUC	2	ACGAATTTACAGTCCGTCCC	TCTCTTTCAAGGAGGCAACG	20	20	50.0	50.0	57.6	57.8	0.0	0.0	0.545005	101318	101377	148-148	1.000000	79.4
trnY-GUA-trnE-UUC_p3	trnY-GUA-trnE-UUC	3	GAATTTACAGTCCGTCCCCA	TCTCTTTCAAGGAGGCAACG	20	20	50.0	50.0	57.2	57.8	0.0	0.0	0.943825	101318	101377	146-146	1.000000	76.3
trnY-GUA-trnE-UUC_p4	trnY-GUA-trnE-UUC	4	CGAATTTACAGTCCGTCCCC	CTCTTTCAAGGAGGCAACGG	20	20	55.0	55.0	58.4	58.8	0.0	0.0	1.191359	101318	101377	146-146	1.000000	74.3
trnY-GUA-trnE-UUC_p5	trnY-GUA-trnE-UUC	5	ACGAATTTACAGTCCGTCCC	CTCTTTCAAGGAGGCAACGG	20	20	50.0	55.0	57.6	58.8	0.0	0.0	1.215036	101318	101377	147-147	1.000000	74.1
psbZ-trnfM-CAU_p1	psbZ-trnfM-CAU	1	CCGGTACATCATTATGGATTGGA	CTTGAGGTCACGGGTTCAAA	23	20	43.5	50.0	58.1	58.0	0.0	4.2	3.132778	106984	107762	831-882	1.000000	62.7
psbZ-trnfM-CAU_p2	psbZ-trnfM-CAU	2	TCCGGTACATCATTATGGATTGG	CTTGAGGTCACGGGTTCAAA	23	20	43.5	50.0	58.1	58.0	0.0	4.2	3.132778	106984	107762	832-883	1.000000	62.7
psbZ-trnfM-CAU_p3	psbZ-trnfM-CAU	3	CCGGTACATCATTATGGATTGGA	TGAGGTCACGGGTTCAAATC	23	20	43.5	50.0	58.1	57.8	0.0	0.0	3.273483	106984	107762	829-880	1.000000	61.6
psbZ-trnfM-CAU_p4	psbZ-trnfM-CAU	4	CCGGTACATCATTATGGATTGGA	TTTTTCGCGGAGTAGAGCAA	23	20	43.5	45.0	58.1	57.8	0.0	6.1	3.252405	106984	107762	876-927	1.000000	61.5
psbZ-trnfM-CAU_p5	psbZ-trnfM-CAU	5	TCCGGTACATCATTATGGATTGG	TTTTTCGCGGAGTAGAGCAA	23	20	43.5	45.0	58.1	57.8	0.0	6.1	3.252405	106984	107762	877-928	1.000000	61.5
trnT-UGU-trnL-UAA_p1	trnT-UGU-trnL-UAA	1	GCGATGCTCTAACCTCTGAG	ACCAATTTCGCCATATCCCC	20	20	55.0	50.0	57.9	58.0	0.0	0.0	0.137364	117170	118163	1041-1052	1.000000	85.3
trnT-UGU-trnL-UAA_p2	trnT-UGU-trnL-UAA	2	GCGATGCTCTAACCTCTGAG	TAGCGTCTACCAATTTCGCC	20	20	55.0	50.0	57.9	58.1	0.0	0.0	0.202173	117170	118163	1049-1060	1.000000	84.8
trnT-UGU-trnL-UAA_p3	trnT-UGU-trnL-UAA	3	GGAATCGAACCGATGACCAT	ACCAATTTCGCCATATCCCC	20	20	50.0	50.0	57.8	58.0	15.5	0.0	0.245117	117170	118163	1073-1084	1.000000	84.3
trnT-UGU-trnL-UAA_p4	trnT-UGU-trnL-UAA	4	GGAATCGAACCGATGACCAT	TAGCGTCTACCAATTTCGCC	20	20	50.0	50.0	57.8	58.1	15.5	0.0	0.309926	117170	118163	1081-1092	1.000000	83.7
trnT-UGU-trnL-UAA_p5	trnT-UGU-trnL-UAA	5	GCGATGCTCTAACCTCTGAG	AGTCCGTAGCGTCTACCAAT	20	20	55.0	50.0	57.9	58.2	0.0	10.2	0.372548	117170	118163	1055-1066	1.000000	83.4