Hotspot

rrn23

Genome · 89499-92315 · 2817 bp

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Pi 0.00355

MarkerSeek score 58.20

Primer available yes

Label rrn23

Feature Metadata

label_name: rrn23
feature_type: rRNA
region: Genome
length_bp: 2817
variable_sites: 10
pi: 0.00355
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 58.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rrn23_p1	ACTTCTGTCGTTTCACGCTT	TAGTAGCACGTTGAAGTGGC	45.0	50.0	58.1	57.9	2944-2945 bp	1.0	79.3
rrn23_p2	TCGGGTTCTCGAATCAGAGA	TAGTAGCACGTTGAAGTGGC	50.0	50.0	57.9	57.9	2963-2964 bp	1.0	78.8
rrn23_p3	TCTCGGGTTCTCGAATCAGA	TAGTAGCACGTTGAAGTGGC	50.0	50.0	57.9	57.9	2965-2966 bp	1.0	78.8
rrn23_p4	CCCAGCTTTTTCTGCGTCTA	TAGTAGCACGTTGAAGTGGC	50.0	50.0	58.2	57.9	2898-2899 bp	1.0	78.3
rrn23_p5	CGGGTTCTCGAATCAGAGAC	TAGTAGCACGTTGAAGTGGC	55.0	50.0	57.8	57.9	2962-2963 bp	1.0	78.2