Hotspot

chlN

Genome · 134535-135929 · 1395 bp

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Pi 0.00358

MarkerSeek score 60.80

Primer available yes

Label chlN

Feature Metadata

label_name: chlN
feature_type: gene
region: Genome
length_bp: 1395
variable_sites: 5
pi: 0.00358
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 60.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
chlN_p1	ACTCGGCAATTTCAAAACAA	TTCTGGAAAAGGGGAGGACA	35.0	50.0	54.6	58.2	1546 bp	1.0	51.7
chlN_p2	ACTCGGCAATTTCAAAACAA	AAGGGGAGGACACTCGAAAT	35.0	50.0	54.6	58.3	1538 bp	1.0	50.3
chlN_p3	ACTCGGCAATTTCAAAACAAA	TTCTGGAAAAGGGGAGGACA	33.3	50.0	55.4	58.2	1546 bp	1.0	50.3
chlN_p4	ACTCGGCAATTTCAAAACAAA	AAGGGGAGGACACTCGAAAT	33.3	50.0	55.4	58.3	1538 bp	1.0	49.0
chlN_p5	ACTCGGCAATTTCAAAACAA	AGGGGAGGACACTCGAAATA	35.0	50.0	54.6	57.5	1537 bp	1.0	48.7