Hotspot

trnR-ACG-ndhB

Genome · 163139-164975 · 1837 bp

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Pi 0.00332

MarkerSeek score 61.40

Primer available yes

Label trnR-ACG-ndhB

Feature Metadata

label_name: trnR-ACG-ndhB
feature_type: igs
region: Genome
length_bp: 1837
variable_sites: 6
pi: 0.00332
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.98
markerseek_score: 61.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnR-ACG-ndhB_p1	AAAAGTGGTTCCTGGAACGG	TCGGGATCACACAGAATACCT	50.0	47.6	58.3	58.3	1974-2043 bp	1.0	68.8
trnR-ACG-ndhB_p2	AAAAGTGGTTCCTGGAACGG	TCGGGATCACACAGAATACC	50.0	50.0	58.3	56.7	1974-2043 bp	1.0	68.7
trnR-ACG-ndhB_p3	AAAAGTGGTTCCTGGAACGG	CGGGATCACACAGAATACCT	50.0	50.0	58.3	56.7	1973-2042 bp	1.0	68.6
trnR-ACG-ndhB_p4	AAAAGTGGTTCCTGGAACGG	CGGGATCACACAGAATACCTT	50.0	47.6	58.3	57.5	1973-2042 bp	1.0	66.5
trnR-ACG-ndhB_p5	AAAAGTGGTTCCTGGAACGG	CCATTATCGGGATCACACAGA	50.0	47.6	58.3	57.2	1980-2049 bp	1.0	64.8