Hotspot

rpl32-trnP-GGG

Genome · 118033-119184 · 1152 bp

Back to Cibotium

Pi 0.00262

MarkerSeek score 60.70

Primer available yes

Label rpl32-trnP-GGG

Feature Metadata

label_name: rpl32-trnP-GGG
feature_type: igs
region: Genome
length_bp: 1152
variable_sites: 3
pi: 0.00262
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 60.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpl32-trnP-GGG_p1	GGAGGGCTAAATCGGTGAAA	CTTGCATGGAGGTAACGGAA	50.0	50.0	57.6	57.9	1360-1363 bp	1.0	76.3
rpl32-trnP-GGG_p2	TGGAGGGCTAAATCGGTGAA	CTTGCATGGAGGTAACGGAA	50.0	50.0	58.7	57.9	1361-1364 bp	1.0	73.9
rpl32-trnP-GGG_p3	GGAGGGCTAAATCGGTGAAA	TACTTGCATGGAGGTAACGG	50.0	50.0	57.6	57.3	1362-1365 bp	1.0	71.7
rpl32-trnP-GGG_p4	TGGAGGGCTAAATCGGTGA	CTTGCATGGAGGTAACGGAA	52.6	50.0	58.0	57.9	1361-1364 bp	1.0	71.6
rpl32-trnP-GGG_p5	GAGGGCTAAATCGGTGAAAGT	CTTGCATGGAGGTAACGGAA	47.6	50.0	58.0	57.9	1359-1362 bp	1.0	71.5