Hotspot

rrn16-rps12

Genome · 96200-103579 · 7380 bp

Back to Cibotium

Pi 0.0035

MarkerSeek score 58.80

Primer available yes

Label rrn16-rps12

Feature Metadata

label_name: rrn16-rps12
feature_type: igs
region: Genome
length_bp: 7380
variable_sites: 24
pi: 0.0035
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.93
markerseek_score: 58.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rrn16-rps12_p1	CATCCCCGTTAGCCTAGAGA	CCTGAGCGATTACGAACCAA	55.0	50.0	58.0	58.0	786-1149 bp	1.0	84.7
rrn16-rps12_p2	CATCCCCGTTAGCCTAGAGA	AAGTAGATCCTGTCCCTCCG	55.0	55.0	58.0	57.9	589-968 bp	1.0	84.2
rrn16-rps12_p3	CATCCCCGTTAGCCTAGAGA	GACGAGCGGGTTAGAAAGAG	55.0	55.0	58.0	58.1	837-1200 bp	1.0	84.0
rrn16-rps12_p4	GGTAGTGCCCTGATTGAGTG	CCTGAGCGATTACGAACCAA	55.0	50.0	58.0	58.0	1006 bp	0.5	59.7
rrn16-rps12_p5	GTAGTGCCCTGATTGAGTGG	CCTGAGCGATTACGAACCAA	55.0	50.0	58.0	58.0	1005 bp	0.5	59.7