Hotspot

rps3-rpl22

Genome · 82585-82671 · 87 bp

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Pi 0.046

MarkerSeek score 78.90

Primer available yes

Label rps3-rpl22

Feature Metadata

label_name: rps3-rpl22
feature_type: igs
region: Genome
length_bp: 87
variable_sites: 4
pi: 0.046
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 78.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rps3-rpl22_p1	GGATGAATCTTTCGCCCCAT	CCGATAAGGAAACCCACCTG	50.0	55.0	58.0	58.0	182 bp	1.0	81.3
rps3-rpl22_p2	AGTGGATGAATCTTTCGCCC	CCGATAAGGAAACCCACCTG	50.0	55.0	58.0	58.0	185 bp	1.0	81.1
rps3-rpl22_p3	ATCTTCCATCTGTGCGAACC	CCGATAAGGAAACCCACCTG	50.0	55.0	58.0	58.0	246 bp	1.0	80.8
rps3-rpl22_p4	GGATGAATCTTTCGCCCCAT	AAAAGGTTTCGTCCTCGGTC	50.0	50.0	58.0	58.1	218 bp	1.0	80.4
rps3-rpl22_p5	ATCTTCCATCTGTGCGAACC	AAAAGGTTTCGTCCTCGGTC	50.0	50.0	58.0	58.1	282 bp	1.0	79.9