Hotspot

rpl20-rps12

Genome · 67371-68404 · 1034 bp

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Pi 0.00291

MarkerSeek score 60.60

Primer available yes

Label rpl20-rps12

Feature Metadata

label_name: rpl20-rps12
feature_type: igs
region: Genome
length_bp: 1034
variable_sites: 3
pi: 0.00291
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 60.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpl20-rps12_p1	CTTGAATGAGCCCCTCGAAA	CGAGGAGTATGTACCAGGGT	50.0	55.0	57.9	57.9	1145-1147 bp	1.0	78.9
rpl20-rps12_p2	CTTGAATGAGCCCCTCGAAA	CTGGGGAGTGGGACAAAATC	50.0	55.0	57.9	58.2	1193-1195 bp	1.0	78.1
rpl20-rps12_p3	CTTGAATGAGCCCCTCGAAA	CAACGGCGAGGAGTATGTAC	50.0	55.0	57.9	58.2	1151-1153 bp	1.0	78.1
rpl20-rps12_p4	TGAATGAGCCCCTCGAAATC	CGAGGAGTATGTACCAGGGT	50.0	55.0	57.7	57.9	1143-1145 bp	1.0	77.1
rpl20-rps12_p5	AATCTTGAATGAGCCCCTCG	CGAGGAGTATGTACCAGGGT	50.0	55.0	57.7	57.9	1148-1150 bp	1.0	77.1