Hotspot

petA-psbJ

Genome · 62330-63044 · 715 bp

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Pi 0.007

MarkerSeek score 52.70

Primer available yes

Label petA-psbJ

Feature Metadata

label_name: petA-psbJ
feature_type: igs
region: Genome
length_bp: 715
variable_sites: 5
pi: 0.007
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 52.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
petA-psbJ_p1	AAAAAGTCCAGCTGGCAGAA	GTTCGGTGGGCATATCCTTT	45.0	50.0	57.9	57.9	802-803 bp	1.0	79.6
petA-psbJ_p2	AAAAGTCCAGCTGGCAGAAA	GTTCGGTGGGCATATCCTTT	45.0	50.0	57.9	57.9	801-802 bp	1.0	79.6
petA-psbJ_p3	TTGAAAAAGTCCAGCTGGCA	GTTCGGTGGGCATATCCTTT	45.0	50.0	58.2	57.9	805-806 bp	1.0	78.4
petA-psbJ_p4	AAAGTCCAGCTGGCAGAAAT	GTTCGGTGGGCATATCCTTT	45.0	50.0	57.7	57.9	800-801 bp	1.0	77.8
petA-psbJ_p5	AAAAGTCCAGCTGGCAGAAA	GAGCCTATTCGGGGTTAGGA	45.0	55.0	57.9	58.3	777-778 bp	1.0	77.7