Hotspot

accD-psaI

Genome · 57811-58404 · 594 bp

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Pi 0.00524

MarkerSeek score 65.20

Primer available yes

Label accD-psaI

Feature Metadata

label_name: accD-psaI
feature_type: igs
region: Genome
length_bp: 594
variable_sites: 3
pi: 0.00524
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.96
markerseek_score: 65.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
accD-psaI_p1	GAAGGGTGTTTTGAGCGAGA	AGGGTAGATAGGACGCTGTC	50.0	55.0	58.1	58.0	664-677 bp	1.0	81.9
accD-psaI_p2	GAAGGGTGTTTTGAGCGAGA	GAGGGTAGATAGGACGCTGT	50.0	55.0	58.1	58.0	665-678 bp	1.0	81.9
accD-psaI_p3	GAAGGGTGTTTTGAGCGAGA	TGTAATTGCCGGAAACACCA	50.0	45.0	58.1	58.0	708-721 bp	1.0	81.7
accD-psaI_p4	CTGAGCCTTATTCCTCAGCC	AGGGTAGATAGGACGCTGTC	55.0	55.0	57.7	58.0	641-654 bp	1.0	80.9
accD-psaI_p5	CTGAGCCTTATTCCTCAGCC	GAGGGTAGATAGGACGCTGT	55.0	55.0	57.7	58.0	642-655 bp	1.0	80.9