Hotspot

ndhG-ndhI

SSC · 112134-112365 · 232 bp

Back to Chusquea

Pi 0.0388

MarkerSeek score 63.60

Primer available yes

Label ndhG-ndhI

Feature Metadata

label_name: ndhG-ndhI
feature_type: igs
region: SSC
length_bp: 232
variable_sites: 9
pi: 0.0388
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 63.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhG-ndhI_p1	TCTCATGTATTGGTCCAGGT	GAGTCGGTTACCAATCTCCA	45.0	50.0	55.5	56.7	378-379 bp	1.0	51.4
ndhG-ndhI_p2	TCTCATGTATTGGTCCAGGT	TGAGTCGGTTACCAATCTCC	45.0	50.0	55.5	56.7	379-380 bp	1.0	51.4
ndhG-ndhI_p3	TCCCCAAATAGCACAAGAATCT	GAGTCGGTTACCAATCTCCA	40.9	50.0	57.4	56.7	397-398 bp	1.0	50.3
ndhG-ndhI_p4	TCCCCAAATAGCACAAGAATCT	TGAGTCGGTTACCAATCTCC	40.9	50.0	57.4	56.7	398-399 bp	1.0	50.3
ndhG-ndhI_p5	TCTCATGTATTGGTCCAGGT	TGAGTCGGTTACCAATCTCCA	45.0	47.6	55.5	58.5	379-380 bp	1.0	50.3