Hotspot

rpl32-trnL-UAG

SSC · 106619-107357 · 739 bp

Back to Chusquea

Pi 0.0176

MarkerSeek score 57.40

Primer available yes

Label rpl32-trnL-UAG

Feature Metadata

label_name: rpl32-trnL-UAG
feature_type: igs
region: SSC
length_bp: 739
variable_sites: 13
pi: 0.0176
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 57.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpl32-trnL-UAG_p1	GGGTTTTTCTGGGCAACAAA	TCCTAAGAGCAGCGTGTCTA	45.0	50.0	57.3	57.9	808-809 bp	1.0	74.8
rpl32-trnL-UAG_p2	GGGTTTTTCTGGGCAACAAA	AGCAGCGTGTCTACCAATTT	45.0	45.0	57.3	57.8	801-802 bp	1.0	74.3
rpl32-trnL-UAG_p3	GGGTTTTTCTGGGCAACAAA	GCTTGAGCACTGCTTCCTAA	45.0	50.0	57.3	58.2	822-823 bp	1.0	74.3
rpl32-trnL-UAG_p4	GGGTTTTTCTGGGCAACAAA	CCTAAGAGCAGCGTGTCTAC	45.0	55.0	57.3	57.8	807-808 bp	1.0	74.2
rpl32-trnL-UAG_p5	GGGTTTTTCTGGGCAACAAA	CTAAGAGCAGCGTGTCTACC	45.0	55.0	57.3	57.8	806-807 bp	1.0	74.2