Hotspot

rpl36-infA

LSC · 76945-77055 · 111 bp

Back to Chusquea

Pi 0.0183

MarkerSeek score 64.80

Primer available yes

Label rpl36-infA

Feature Metadata

label_name: rpl36-infA
feature_type: igs
region: LSC
length_bp: 111
variable_sites: 2
pi: 0.0183
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.98
markerseek_score: 64.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpl36-infA_p1	AACTCTAATTCGTCCACGCC	ATTCGAAGCGTACCGAAGAC	50.0	50.0	58.0	58.1	270-272 bp	1.0	80.4
rpl36-infA_p2	AACTCTAATTCGTCCACGCC	GATTCGAAGCGTACCGAAGA	50.0	50.0	58.0	57.8	271-273 bp	1.0	79.7
rpl36-infA_p3	AACTCTAATTCGTCCACGCC	CCCATAAGGATTCGAAGCGT	50.0	50.0	58.0	57.8	279-281 bp	1.0	79.2
rpl36-infA_p4	AACTCTAATTCGTCCACGCC	TAAGGATTCGAAGCGTACCG	50.0	50.0	58.0	57.5	275-277 bp	1.0	77.3
rpl36-infA_p5	AACTCTAATTCGTCCACGCC	CCCCATAAGGATTCGAAGCG	50.0	55.0	58.0	58.5	280-282 bp	1.0	77.2