Hotspot

rbcL-psaI

LSC · 56752-58049 · 1298 bp

Back to Chusquea

Pi 0.0146

MarkerSeek score 55.60

Primer available yes

Label rbcL-psaI

Feature Metadata

label_name: rbcL-psaI
feature_type: igs
region: LSC
length_bp: 1298
variable_sites: 19
pi: 0.0146
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 55.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rbcL-psaI_p1	CGAGTTCGAGCCGGTAGATA	CCATTGCAATTGCCGGAAAT	55.0	45.0	58.8	58.0	1401-1402 bp	1.0	74.7
rbcL-psaI_p2	CGAGTTCGAGCCGGTAGATA	AAGAAGCCATTGCAATTGCC	55.0	45.0	58.8	57.9	1407-1408 bp	1.0	74.1
rbcL-psaI_p3	AGTTCGAGCCGGTAGATAAAC	CCATTGCAATTGCCGGAAAT	47.6	45.0	57.6	58.0	1399-1400 bp	1.0	69.5
rbcL-psaI_p4	GTTCGAGCCGGTAGATAAACT	CCATTGCAATTGCCGGAAAT	47.6	45.0	57.6	58.0	1398-1399 bp	1.0	69.5
rbcL-psaI_p5	GAGTTCGAGCCGGTAGATAA	CCATTGCAATTGCCGGAAAT	50.0	45.0	56.6	58.0	1400-1401 bp	1.0	69.4