Hotspot

ndhC-trnV-UAC

LSC · 50931-51577 · 647 bp

Back to Chusquea

Pi 0.00618

MarkerSeek score 62.30

Primer available yes

Label ndhC-trnV-UAC

Feature Metadata

label_name: ndhC-trnV-UAC
feature_type: igs
region: LSC
length_bp: 647
variable_sites: 4
pi: 0.00618
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 62.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhC-trnV-UAC_p1	CTAACCGGGGCTAAAAGTCC	GGTTCGAATCCGTATAGCCC	55.0	55.0	58.0	57.9	773-887 bp	1.0	79.5
ndhC-trnV-UAC_p2	CTAACCGGGGCTAAAAGTCC	CGGTTCGAATCCGTATAGCC	55.0	55.0	58.0	58.3	774-888 bp	1.0	77.7
ndhC-trnV-UAC_p3	TAACCGGGGCTAAAAGTCCT	GGTTCGAATCCGTATAGCCC	50.0	55.0	58.3	57.9	772-886 bp	1.0	77.1
ndhC-trnV-UAC_p4	CACTAACCGGGGCTAAAAGT	GGTTCGAATCCGTATAGCCC	50.0	55.0	57.5	57.9	775-889 bp	1.0	75.9
ndhC-trnV-UAC_p5	TAACCGGGGCTAAAAGTCCT	CGGTTCGAATCCGTATAGCC	50.0	55.0	58.3	58.3	773-887 bp	1.0	75.2