Hotspot

trnT-UGU-trnL-UAA

LSC · 46904-47604 · 701 bp

Back to Chusquea

Pi 0.0118

MarkerSeek score 63.20

Primer available yes

Label trnT-UGU-trnL-UAA

Feature Metadata

label_name: trnT-UGU-trnL-UAA
feature_type: igs
region: LSC
length_bp: 701
variable_sites: 8
pi: 0.0118
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.97
markerseek_score: 63.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnT-UGU-trnL-UAA_p1	ATTTGAACCGATGACCCTCG	CCGTAGCGTCTACCGATTTC	50.0	55.0	58.0	58.2	775-792 bp	1.0	80.8
trnT-UGU-trnL-UAA_p2	CGATGACCCTCGCATTACAA	CCGTAGCGTCTACCGATTTC	50.0	55.0	58.1	58.2	767-784 bp	1.0	80.5
trnT-UGU-trnL-UAA_p3	GCGATGCTCTAACCTCTGAG	CCGTAGCGTCTACCGATTTC	55.0	55.0	57.9	58.2	745-762 bp	1.0	80.0
trnT-UGU-trnL-UAA_p4	ATTTGAACCGATGACCCTCG	CGTCTACCGATTTCGCCATA	50.0	50.0	58.0	57.6	769-786 bp	1.0	79.5
trnT-UGU-trnL-UAA_p5	CGATGACCCTCGCATTACAA	CGTCTACCGATTTCGCCATA	50.0	50.0	58.1	57.6	761-778 bp	1.0	79.2