Hotspot

atpH-atpF

LSC · 33389-33842 · 454 bp

Back to Chusquea

Pi 0.0154

MarkerSeek score 64.60

Primer available yes

Label atpH-atpF

Feature Metadata

label_name: atpH-atpF
feature_type: igs
region: LSC
length_bp: 454
variable_sites: 7
pi: 0.0154
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 64.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
atpH-atpF_p1	AGTTGTGGCACTAGCACTTT	GGATGGCCAGTGAGCTAAAA	45.0	50.0	57.7	57.9	545 bp	1.0	77.2
atpH-atpF_p2	AGTTGTGGCACTAGCACTTT	ATTAAGCCCGAAACTCCCAG	45.0	50.0	57.7	57.6	566 bp	1.0	74.8
atpH-atpF_p3	CTAGTTGTGGCACTAGCACT	GGATGGCCAGTGAGCTAAAA	50.0	50.0	57.2	57.9	547 bp	1.0	74.0
atpH-atpF_p4	CTAGTTGTGGCACTAGCACT	ATTAAGCCCGAAACTCCCAG	50.0	50.0	57.2	57.6	568 bp	1.0	71.6
atpH-atpF_p5	CTAGTTGTGGCACTAGCACTT	GGATGGCCAGTGAGCTAAAA	47.6	50.0	58.0	57.9	547 bp	1.0	71.6