Hotspot

trnS-GCU-psbD

LSC · 8006-8989 · 984 bp

Back to Chusquea

Pi 0.00916

MarkerSeek score 57.20

Primer available yes

Label trnS-GCU-psbD

Feature Metadata

label_name: trnS-GCU-psbD
feature_type: igs
region: LSC
length_bp: 984
variable_sites: 9
pi: 0.00916
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 57.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnS-GCU-psbD_p1	ATTAGCAATCCGCCGCTTTA	GCCGGACCATCCTACAAAAA	45.0	50.0	58.0	58.2	1128-1129 bp	1.0	79.0
trnS-GCU-psbD_p2	ATTAGCAATCCGCCGCTTTA	ACAAAAACGAAACGGTCCCT	45.0	45.0	58.0	58.2	1115-1116 bp	1.0	78.4
trnS-GCU-psbD_p3	GCTTTAGTCCACTCAGCCAT	GCCGGACCATCCTACAAAAA	50.0	50.0	57.6	58.2	1114-1115 bp	1.0	76.1
trnS-GCU-psbD_p4	ATTAGCAATCCGCCGCTTTA	GAAACGGTCCCTTCGTAACC	45.0	55.0	58.0	58.6	1107-1108 bp	1.0	75.9
trnS-GCU-psbD_p5	GCTTTAGTCCACTCAGCCAT	ACAAAAACGAAACGGTCCCT	50.0	45.0	57.6	58.2	1101-1102 bp	1.0	75.5