Hotspot

petA

LSC · 61940-62902 · 963 bp

Back to Chuquiraga

Pi 0.00104
MarkerSeek score 31.60
Primer available yes
Label petA

Feature Metadata

label_name
petA
feature_type
gene
region
LSC
length_bp
963
variable_sites
2
pi
0.00104
unique_haplotype_count
2
primer_available
yes
alignment_reliability
1.00
markerseek_score
31.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
petA_p1 CTCTTGGGCTCAGGAATTCC GGCGTAAGAACTCAACGGAT 55.0 50.0 58.0 58.0 2210-2231 bp 1.0 80.3
petA_p2 CTCTTGGGCTCAGGAATTCC AAGACGAATAACCCCTCCCA 55.0 50.0 58.0 58.0 1851-1875 bp 1.0 79.9
petA_p3 CTCTTGGGCTCAGGAATTCC ACGAATAACCCCTCCCAGAA 55.0 50.0 58.0 58.0 1848-1872 bp 1.0 79.9
petA_p4 CTCTTGGGCTCAGGAATTCC GCTAGGAATGTGGTCCAAGG 55.0 55.0 58.0 58.0 1631 bp 1.0 79.4
petA_p5 CTCTTGGGCTCAGGAATTCC GGCTAGGAATGTGGTCCAAG 55.0 55.0 58.0 58.0 1632 bp 0.75 69.2