Hotspot

ndhF

SSC · 108572-110809 · 2238 bp

Back to Chuquiraga

Pi 0.0029

MarkerSeek score 44.20

Primer available yes

Label ndhF

Feature Metadata

label_name: ndhF
feature_type: gene
region: SSC
length_bp: 2238
variable_sites: 12
pi: 0.0029
unique_haplotype_count: 4
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 44.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhF_p1	CTAGCACTGGGTAGACCTCA	GCCGATGACCTTAGTTACTCC	55.0	52.4	57.9	57.9	2848 bp	0.25	40.1
ndhF_p2	CTAGCACTGGGTAGACCTCA	GGCCGATGACCTTAGTTACTC	55.0	52.4	57.9	57.9	2849 bp	0.25	40.1
ndhF_p3	ATTACGGGACAGCTCCTGAT	GCCGATGACCTTAGTTACTCC	50.0	52.4	58.2	57.9	2899 bp	0.25	39.6
ndhF_p4	ATTACGGGACAGCTCCTGAT	GGCCGATGACCTTAGTTACTC	50.0	52.4	58.2	57.9	2900 bp	0.25	39.6
ndhF_p5	TCTCTTCCTTCTCCGAGCTC	GCCGATGACCTTAGTTACTCC	55.0	52.4	58.2	57.9	2978 bp	0.25	39.3