Hotspot

ndhA

SSC · 118451-120597 · 2147 bp

Back to Chuquiraga

Pi 0.00295

MarkerSeek score 59.80

Primer available yes

Label ndhA

Feature Metadata

label_name: ndhA
feature_type: gene
region: SSC
length_bp: 2147
variable_sites: 12
pi: 0.00295
unique_haplotype_count: 4
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 59.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhA_p1	TGTTTGAGACAAGTCGTGAA	CCACCGGGGTTTATTAATTTGC	40.0	45.5	55.2	58.2	2300-2304 bp	1.0	40.7
ndhA_p2	TTGTTTGAGACAAGTCGTGA	CCACCGGGGTTTATTAATTTGC	40.0	45.5	55.2	58.2	2301-2305 bp	1.0	40.7
ndhA_p3	TTGTTTGAGACAAGTCGTGAA	CCACCGGGGTTTATTAATTTGC	38.1	45.5	56.0	58.2	2301-2305 bp	1.0	40.7
ndhA_p4	TGTTTGAGACAAGTCGTGAA	CACCGGGGTTTATTAATTTGCA	40.0	40.9	55.2	57.5	2299-2303 bp	1.0	40.7
ndhA_p5	TTGTTTGAGACAAGTCGTGA	CACCGGGGTTTATTAATTTGCA	40.0	40.9	55.2	57.5	2300-2304 bp	1.0	40.7