Hotspot

trnL-CAA-ycf2

IRa · 142946-143341 · 396 bp

Back to Chuquiraga

Pi 0.00884
MarkerSeek score 52.30
Primer available yes
Label trnL-CAA-ycf2

Feature Metadata

label_name
trnL-CAA-ycf2
feature_type
igs
region
IRa
length_bp
396
variable_sites
7
pi
0.00884
unique_haplotype_count
3
primer_available
yes
alignment_reliability
1.00
markerseek_score
52.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnL-CAA-ycf2_p1 AAATGGTAGACACGCGAGAC GCTTTTCCCGGATGAAATGC 50.0 50.0 58.0 58.1 538-543 bp 1.0 81.1
trnL-CAA-ycf2_p2 TGAAATGGTAGACACGCGAG GCTTTTCCCGGATGAAATGC 50.0 50.0 58.0 58.1 540-545 bp 1.0 81.1
trnL-CAA-ycf2_p3 GCCTTGGTGGTGAAATGGTA GCTTTTCCCGGATGAAATGC 50.0 50.0 58.1 58.1 550-555 bp 1.0 80.5
trnL-CAA-ycf2_p4 AAATGGTAGACACGCGAGAC ATGGCTTTTCCCGGATGAAA 50.0 45.0 58.0 57.8 541-546 bp 1.0 79.9
trnL-CAA-ycf2_p5 AAATGGTAGACACGCGAGAC TGGCTTTTCCCGGATGAAAT 50.0 45.0 58.0 57.8 540-545 bp 1.0 79.9