Hotspot

rpl20-rps12

LSC · 68652-69377 · 726 bp

Back to Chuquiraga

Pi 0.0023

MarkerSeek score 63.10

Primer available yes

Label rpl20-rps12

Feature Metadata

label_name: rpl20-rps12
feature_type: igs
region: LSC
length_bp: 726
variable_sites: 3
pi: 0.0023
unique_haplotype_count: 4
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 63.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpl20-rps12_p1	TCTCCGAGCGATATATCCCC	TGTATGTGCGACTCGTTCAG	55.0	50.0	57.9	58.0	738-799 bp	1.0	83.7
rpl20-rps12_p2	TCTCCGAGCGATATATCCCC	ATGTGCGACTCGTTCAGATC	55.0	50.0	57.9	58.1	735-796 bp	1.0	83.2
rpl20-rps12_p3	TCTCCGAGCGATATATCCCC	GTGCGACTCGTTCAGATCAT	55.0	50.0	57.9	58.1	733-794 bp	1.0	83.2
rpl20-rps12_p4	TCTCCGAGCGATATATCCCC	TGCGACTCGTTCAGATCATG	55.0	50.0	57.9	58.1	732-793 bp	1.0	83.2
rpl20-rps12_p5	TCTCCGAGCGATATATCCCC	ACTAGGGTGTATGTGCGACT	55.0	50.0	57.9	58.2	745-806 bp	1.0	82.7