Hotspot

accD-psaI

LSC · 58417-59081 · 665 bp

Back to Chuquiraga

Pi 0.00478

MarkerSeek score 63.90

Primer available yes

Label accD-psaI

Feature Metadata

label_name: accD-psaI
feature_type: igs
region: LSC
length_bp: 665
variable_sites: 6
pi: 0.00478
unique_haplotype_count: 4
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 63.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
accD-psaI_p1	TTCGAGAAGGGCTTATTCGA	AAAGAAGCCATTGCGATTGC	45.0	45.0	56.4	58.0	836-854 bp	1.0	68.6
accD-psaI_p2	TCGAGAAGGGCTTATTCGATC	AAAGAAGCCATTGCGATTGC	47.6	45.0	57.4	58.0	835-853 bp	1.0	68.4
accD-psaI_p3	CGAGAAGGGCTTATTCGATCT	AAAGAAGCCATTGCGATTGC	47.6	45.0	57.3	58.0	834-852 bp	1.0	68.4
accD-psaI_p4	TCGAGAAGGGCTTATTCGAT	AAAGAAGCCATTGCGATTGC	45.0	45.0	56.1	58.0	835-853 bp	1.0	66.7
accD-psaI_p5	TTCGAGAAGGGCTTATTCGA	TTGCGATTGCCGGAAATACT	45.0	45.0	56.4	58.3	826-844 bp	1.0	66.5