Hotspot

ndhC-trnV-UAC

LSC · 50633-51081 · 449 bp

Back to Chuquiraga

Pi 0.00528

MarkerSeek score 60.10

Primer available yes

Label ndhC-trnV-UAC

Feature Metadata

label_name: ndhC-trnV-UAC
feature_type: igs
region: LSC
length_bp: 449
variable_sites: 4
pi: 0.00528
unique_haplotype_count: 4
primer_available: yes
alignment_reliability: 0.98
markerseek_score: 60.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhC-trnV-UAC_p1	GTCCTTTGCTAATCGGAGCT	AGAAGGTCTACGGTTCGAGT	50.0	50.0	57.7	57.8	573-1053 bp	1.0	79.8
ndhC-trnV-UAC_p2	CGGTCCTTTGCTAATCGGAG	AGAAGGTCTACGGTTCGAGT	55.0	50.0	58.4	57.8	575-1055 bp	1.0	78.9
ndhC-trnV-UAC_p3	CTCCGGTCCTTTGCTAATCG	AGAAGGTCTACGGTTCGAGT	55.0	50.0	58.4	57.8	578-1058 bp	1.0	78.9
ndhC-trnV-UAC_p4	GTCCTTTGCTAATCGGAGCT	GAAGGTCTACGGTTCGAGTC	50.0	55.0	57.7	57.5	572-1052 bp	1.0	77.0
ndhC-trnV-UAC_p5	GGTCCTTTGCTAATCGGAGC	AGAAGGTCTACGGTTCGAGT	55.0	50.0	58.7	57.8	574-1054 bp	1.0	76.7