Hotspot

trnC-GCA-petN

LSC · 28558-29386 · 829 bp

Back to Chuquiraga

Pi 0.00543

MarkerSeek score 61.90

Primer available yes

Label trnC-GCA-petN

Feature Metadata

label_name: trnC-GCA-petN
feature_type: igs
region: LSC
length_bp: 829
variable_sites: 9
pi: 0.00543
unique_haplotype_count: 4
primer_available: yes
alignment_reliability: 0.91
markerseek_score: 61.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnC-GCA-petN_p1	TTCTCCTATCGTTTTGGCGG	AGCCCAAGCGAGACTTACTA	50.0	50.0	58.0	58.2	838-942 bp	1.0	83.4
trnC-GCA-petN_p2	TTTCCCCAGTTCAAATCCGG	AGCCCAAGCGAGACTTACTA	50.0	50.0	58.1	58.2	781-885 bp	1.0	83.0
trnC-GCA-petN_p3	GGGGGACTGCAAATCCTTTT	AGCCCAAGCGAGACTTACTA	50.0	50.0	58.4	58.2	799-903 bp	1.0	80.8
trnC-GCA-petN_p4	TTCTCCTATCGTTTTGGCGG	GCCCAAGCGAGACTTACTAT	50.0	50.0	58.0	57.1	837-941 bp	1.0	77.5
trnC-GCA-petN_p5	TTCTCCTATCGTTTTGGCGG	ATTAAAGCAGCCCAAGCGAG	50.0	50.0	58.0	58.9	846-950 bp	1.0	77.4