Hotspot

atpH-atpI

LSC · 13910-14970 · 1061 bp

Back to Chuquiraga

Pi 0.00377

MarkerSeek score 48.20

Primer available yes

Label atpH-atpI

Feature Metadata

label_name: atpH-atpI
feature_type: igs
region: LSC
length_bp: 1061
variable_sites: 8
pi: 0.00377
unique_haplotype_count: 4
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 48.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
atpH-atpI_p1	CTCGCGATACCTTCTACAGC	CACTCTAGCTGTTCGGAACC	55.0	55.0	58.0	58.0	1762-1766 bp	1.0	80.3
atpH-atpI_p2	CTCGCGATACCTTCTACAGC	CCACTCTAGCTGTTCGGAAC	55.0	55.0	58.0	58.0	1763-1767 bp	1.0	80.3
atpH-atpI_p3	CTCGCGATACCTTCTACAGC	TGCTTTGGCTTTACTCACGT	55.0	45.0	58.0	58.0	1510-1514 bp	1.0	80.2
atpH-atpI_p4	CTCGCGATACCTTCTACAGC	GAGAAGAATACGGCCCTTGG	55.0	55.0	58.0	58.0	1661-1665 bp	1.0	80.0
atpH-atpI_p5	CTCGCGATACCTTCTACAGC	GGAGAAGAATACGGCCCTTG	55.0	55.0	58.0	58.0	1662-1666 bp	1.0	80.0