Hotspot

rps16-trnQ-UUG

LSC · 6285-7233 · 949 bp

Back to Chuquiraga

Pi 0.00334

MarkerSeek score 62.20

Primer available yes

Label rps16-trnQ-UUG

Feature Metadata

label_name: rps16-trnQ-UUG
feature_type: igs
region: LSC
length_bp: 949
variable_sites: 6
pi: 0.00334
unique_haplotype_count: 4
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 62.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rps16-trnQ-UUG_p1	AAGTCGCACGTTGCTTTCTA	GAGGTTCGAATCCTTCCGTC	45.0	55.0	58.1	58.1	1014-1021 bp	1.0	79.5
rps16-trnQ-UUG_p2	TCATGTCCTTCAAGTCGCAC	GAGGTTCGAATCCTTCCGTC	50.0	55.0	58.2	58.1	1025-1032 bp	1.0	79.0
rps16-trnQ-UUG_p3	CACACCGGATCATGTCCTTC	GAGGTTCGAATCCTTCCGTC	55.0	55.0	58.3	58.1	1034-1041 bp	1.0	77.9
rps16-trnQ-UUG_p4	CACCGGATCATGTCCTTCAA	GAGGTTCGAATCCTTCCGTC	50.0	55.0	57.6	58.1	1032-1039 bp	1.0	77.4
rps16-trnQ-UUG_p5	CCAAGAGCACCTTCATTCCT	GAGGTTCGAATCCTTCCGTC	50.0	55.0	57.5	58.1	1085-1092 bp	1.0	76.5