Hotspot

rps15

SSC · 124134-124406 · 273 bp

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Pi 0.00488

MarkerSeek score 28.60

Primer available yes

Label rps15

Feature Metadata

label_name: rps15
feature_type: gene
region: SSC
length_bp: 273
variable_sites: 2
pi: 0.00488
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 28.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rps15_p1	GGCTCAGCTCCAGCATAATT	TTCTTTGGCCCAATTACCGA	50.0	45.0	58.0	57.4	1193 bp	1.0	75.7
rps15_p2	CCTCTCCACCAATAATGCCC	TTCTTTGGCCCAATTACCGA	55.0	45.0	58.0	57.4	1502 bp	1.0	75.6
rps15_p3	TGGCGGCTTCGAATAAATCA	TTCTTTGGCCCAATTACCGA	45.0	45.0	58.0	57.4	1310 bp	1.0	75.5
rps15_p4	AACACCATGCATTGATGGGT	TTCTTTGGCCCAATTACCGA	45.0	45.0	58.1	57.4	940 bp	1.0	75.5
rps15_p5	GAAGCTATACGGCTCAGCTC	TTCTTTGGCCCAATTACCGA	55.0	45.0	57.9	57.4	1203 bp	1.0	75.4