Hotspot

psbA

LSC · 125-1186 · 1062 bp

Back to Chuniophoenix

Pi 0.000628

MarkerSeek score 35.50

Primer available yes

Label psbA

Feature Metadata

label_name: psbA
feature_type: gene
region: LSC
length_bp: 1062
variable_sites: 1
pi: 0.000628
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 35.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psbA_p1	TTGCTCCATTCCTTCAACGA	GTGTGCTTGGGAGTTCTTGA	45.0	50.0	57.4	58.0	1151 bp	1.0	75.3
psbA_p2	TTGCTCCATTCCTTCAACGA	ATACGTGTGCTTGGGAGTTC	45.0	50.0	57.4	57.9	1155 bp	1.0	74.8
psbA_p3	TGCTCCATTCCTTCAACGAT	GTGTGCTTGGGAGTTCTTGA	45.0	50.0	57.2	58.0	1150 bp	1.0	73.5
psbA_p4	TGCTCCATTCCTTCAACGAT	ATACGTGTGCTTGGGAGTTC	45.0	50.0	57.2	57.9	1154 bp	1.0	73.0
psbA_p5	TGCTCCATTCCTTCAACGATT	GTGTGCTTGGGAGTTCTTGA	42.9	50.0	57.9	58.0	1150 bp	1.0	71.2