Hotspot

psaC

SSC · 117737-117982 · 246 bp

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Pi 0

MarkerSeek score 19.90

Primer available yes

Label psaC

Feature Metadata

label_name: psaC
feature_type: gene
region: SSC
length_bp: 246
variable_sites: 0
pi: 0
unique_haplotype_count: 1
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 19.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psaC_p1	CCCCCTGCTGTGTACAAAAT	CTTCGTCTGGTGTCGACAAT	50.0	50.0	58.1	57.9	990 bp	1.0	79.5
psaC_p2	TGTAGCCGAGTAGAGACGTT	CTTCGTCTGGTGTCGACAAT	50.0	50.0	57.9	57.9	964 bp	1.0	79.3
psaC_p3	TTGTAGCCGAGTAGAGACGT	CTTCGTCTGGTGTCGACAAT	50.0	50.0	57.9	57.9	965 bp	1.0	79.3
psaC_p4	GTGAATTTCGCGTAACTGGC	CTTCGTCTGGTGTCGACAAT	50.0	50.0	58.1	57.9	803 bp	1.0	79.3
psaC_p5	GTTTTGACCAGAAAAGCCCG	CTTCGTCTGGTGTCGACAAT	50.0	50.0	57.9	57.9	448 bp	1.0	79.1