Hotspot

petD

LSC · 77285-78529 · 1245 bp

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Pi 0.00107

MarkerSeek score 42.70

Primer available yes

Label petD

Feature Metadata

label_name: petD
feature_type: gene
region: LSC
length_bp: 1245
variable_sites: 2
pi: 0.00107
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 42.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
petD_p1	ACTTCTCTTCAACTCCGAAGT	TGGCACGATCCGTATTTTCC	42.9	50.0	56.6	58.3	1411-1425 bp	1.0	58.3
petD_p2	TGGATACTTCTCTTCAACTCCG	TGGCACGATCCGTATTTTCC	45.5	50.0	57.3	58.3	1416-1430 bp	1.0	55.6
petD_p3	ACTTCTCTTCAACTCCGAAGT	AATCTTTGGCACGATCCGTA	42.9	45.0	56.6	57.3	1417-1431 bp	1.0	55.6
petD_p4	ACTTCTCTTCAACTCCGAAGT	TAATCTTTGGCACGATCCGT	42.9	45.0	56.6	57.3	1418-1432 bp	1.0	55.6
petD_p5	ACTTCTCTTCAACTCCGAAGT	TCTTTGGCACGATCCGTATT	42.9	45.0	56.6	57.3	1415-1429 bp	1.0	55.6