Hotspot

ccsA

SSC · 114922-115890 · 969 bp

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Pi 0.000688

MarkerSeek score 35.80

Primer available yes

Label ccsA

Feature Metadata

label_name: ccsA
feature_type: gene
region: SSC
length_bp: 969
variable_sites: 1
pi: 0.000688
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 35.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ccsA_p1	GAGTGGCGGCATCCTATAAA	ACTTTCGTTATATATGGGACGA	50.0	36.4	57.5	54.6	1107 bp	1.0	40.1
ccsA_p2	GAGTGGCGGCATCCTATAAA	GCAAACTTTCGTTATATATGGGACG	50.0	40.0	57.5	58.6	1111 bp	1.0	40.1
ccsA_p3	GAGTGGCGGCATCCTATAAA	AACTTTCGTTATATATGGGACGA	50.0	34.8	57.5	55.3	1108 bp	1.0	40.1
ccsA_p4	GAGTGGCGGCATCCTATAAA	CAAACTTTCGTTATATATGGGACGA	50.0	36.0	57.5	57.3	1110 bp	1.0	40.1
ccsA_p5	GAGTGGCGGCATCCTATAAA	GCAAACTTTCGTTATATATGGGAC	50.0	37.5	57.5	56.2	1111 bp	1.0	40.1