Hotspot

psaC-ndhE

SSC · 117983-118682 · 700 bp

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Pi 0.00205

MarkerSeek score 53.20

Primer available yes

Label psaC-ndhE

Feature Metadata

label_name: psaC-ndhE
feature_type: igs
region: SSC
length_bp: 700
variable_sites: 1
pi: 0.00205
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 0.46
markerseek_score: 53.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psaC-ndhE_p1	ACATCCGTCCCAAGGTATCA	TGTTATAGCTGTTGCGGCTG	50.0	50.0	58.1	58.6	539-914 bp	1.0	78.1
psaC-ndhE_p2	CAATACATCTGTGGGGCAGG	TGTTATAGCTGTTGCGGCTG	55.0	50.0	58.3	58.6	515-890 bp	1.0	76.5
psaC-ndhE_p3	CCAATACATCTGTGGGGCAG	TGTTATAGCTGTTGCGGCTG	55.0	50.0	58.3	58.6	516-891 bp	1.0	76.5
psaC-ndhE_p4	CAGGCTCGTACACATTGAGT	TGTTATAGCTGTTGCGGCTG	50.0	50.0	57.6	58.6	499-874 bp	1.0	76.1
psaC-ndhE_p5	AGCTTTACATCCGTCCCAAG	TGTTATAGCTGTTGCGGCTG	50.0	50.0	57.6	58.6	545-920 bp	1.0	75.8