Hotspot

ndhF-rpl32

SSC · 113345-113780 · 436 bp

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Pi 0.0107
MarkerSeek score 46.20
Primer available yes
Label ndhF-rpl32

Feature Metadata

label_name
ndhF-rpl32
feature_type
igs
region
SSC
length_bp
436
variable_sites
3
pi
0.0107
unique_haplotype_count
3
primer_available
yes
alignment_reliability
0.50
markerseek_score
46.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
ndhF-rpl32_p1 TTGTTGCTGTCGGAATAAGT TTCTTCCAAATATTCCTACGAA 40.0 31.8 55.3 52.9 381-601 bp 1.0 44.5
ndhF-rpl32_p2 TTGTTGCTGTCGGAATAAGT TTTCTTCCAAATATTCCTACGA 40.0 31.8 55.3 52.9 382-602 bp 1.0 44.5
ndhF-rpl32_p3 TTGTTGCTGTCGGAATAAGT TTTCTTCCAAATATTCCTACGAA 40.0 30.4 55.3 53.8 382-602 bp 1.0 44.5
ndhF-rpl32_p4 TTGTTGCTGTCGGAATAAGT TTTTCTTCCAAATATTCCTACGA 40.0 30.4 55.3 53.8 383-603 bp 1.0 44.5
ndhF-rpl32_p5 TTTGTTGCTGTCGGAATAAGT TTCTTCCAAATATTCCTACGAA 38.1 31.8 56.1 52.9 382-602 bp 1.0 44.5