Hotspot

petD-rpoA

LSC · 78530-78710 · 181 bp

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Pi 0.0184

MarkerSeek score 50.70

Primer available yes

Label petD-rpoA

Feature Metadata

label_name: petD-rpoA
feature_type: igs
region: LSC
length_bp: 181
variable_sites: 5
pi: 0.0184
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 50.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
petD-rpoA_p1	TCGGTACCGTAGTAGCTCTT	ACACTCTACAGAAGTATTTCGCA	50.0	39.1	57.3	57.6	356-357 bp	1.0	48.4
petD-rpoA_p2	ATCGGTACCGTAGTAGCTCT	ACACTCTACAGAAGTATTTCGCA	50.0	39.1	57.1	57.6	357-358 bp	1.0	46.6
petD-rpoA_p3	TCGGTACCGTAGTAGCTCTTT	ACACTCTACAGAAGTATTTCGCA	47.6	39.1	58.0	57.6	356-357 bp	1.0	46.0
petD-rpoA_p4	CGGTACCGTAGTAGCTCTTTG	ACACTCTACAGAAGTATTTCGCA	52.4	39.1	57.9	57.6	355-356 bp	1.0	45.5
petD-rpoA_p5	ATCGGTACCGTAGTAGCTCTT	ACACTCTACAGAAGTATTTCGCA	47.6	39.1	57.8	57.6	357-358 bp	1.0	44.4