Hotspot

psbC-trnS-UGA

LSC · 35596-35731 · 136 bp

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Pi 0.0343

MarkerSeek score 54.40

Primer available yes

Label psbC-trnS-UGA

Feature Metadata

label_name: psbC-trnS-UGA
feature_type: igs
region: LSC
length_bp: 136
variable_sites: 7
pi: 0.0343
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 54.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psbC-trnS-UGA_p1	CTGTTCTTTCCATGACCCCC	GGTTGATAGCTCCGGTCTTG	55.0	55.0	58.2	58.1	243 bp	1.0	80.6
psbC-trnS-UGA_p2	GCAGCAGGGTTTGAAAAAGG	GGTTGATAGCTCCGGTCTTG	50.0	55.0	58.1	58.1	283 bp	1.0	80.5
psbC-trnS-UGA_p3	CCTGTTCTTTCCATGACCCC	GGTTGATAGCTCCGGTCTTG	55.0	55.0	58.2	58.1	244 bp	1.0	80.5
psbC-trnS-UGA_p4	CTGTTCTTTCCATGACCCCC	GATGGCCGAGTGGTTGATAG	55.0	55.0	58.2	58.1	254 bp	1.0	79.9
psbC-trnS-UGA_p5	GCAGCAGGGTTTGAAAAAGG	GATGGCCGAGTGGTTGATAG	50.0	55.0	58.1	58.1	294 bp	1.0	79.8