Hotspot

trnS-GCU-trnG-GCC

LSC · 7856-8868 · 1013 bp

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Pi 0.00413

MarkerSeek score 46.70

Primer available yes

Label trnS-GCU-trnG-GCC

Feature Metadata

label_name: trnS-GCU-trnG-GCC
feature_type: igs
region: LSC
length_bp: 1013
variable_sites: 4
pi: 0.00413
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 0.64
markerseek_score: 46.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnS-GCU-trnG-GCC_p1	ATTAGCAATCCGCCGCTTTA	ACGAATCACACTTTTACCACT	45.0	38.1	58.0	55.5	720-1087 bp	1.0	55.8
trnS-GCU-trnG-GCC_p2	ATTAGCAATCCGCCGCTTTA	AGAACGAATCACACTTTTACCAC	45.0	39.1	58.0	57.3	723-1090 bp	1.0	54.7
trnS-GCU-trnG-GCC_p3	ATTAGCAATCCGCCGCTTTA	GAACGAATCACACTTTTACCACT	45.0	39.1	58.0	57.3	722-1089 bp	1.0	54.7
trnS-GCU-trnG-GCC_p4	AGAAGAAGCGGAGAGAGAGG	ACGAATCACACTTTTACCACT	55.0	38.1	58.2	55.5	776-1143 bp	1.0	54.2
trnS-GCU-trnG-GCC_p5	ATTAGCAATCCGCCGCTTTA	AACGAATCACACTTTTACCACT	45.0	36.4	58.0	56.2	721-1088 bp	1.0	53.9