Hotspot

psbK-psbI

LSC · 7120-7520 · 401 bp

Back to Chuniophoenix

Pi 0.00831

MarkerSeek score 52.20

Primer available yes

Label psbK-psbI

Feature Metadata

label_name: psbK-psbI
feature_type: igs
region: LSC
length_bp: 401
variable_sites: 5
pi: 0.00831
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 52.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psbK-psbI_p1	GTTTGGCAAGCTGCTGTAAG	AGGATTACGCCCTGGATCAT	50.0	50.0	57.9	58.3	478-527 bp	1.0	82.2
psbK-psbI_p2	TGGCAAGCTGCTGTAAGTTT	AGGATTACGCCCTGGATCAT	45.0	50.0	58.3	58.3	475-524 bp	1.0	80.2
psbK-psbI_p3	TTGGCAAGCTGCTGTAAGTT	AGGATTACGCCCTGGATCAT	45.0	50.0	58.3	58.3	476-525 bp	1.0	80.2
psbK-psbI_p4	TTTGGCAAGCTGCTGTAAGT	AGGATTACGCCCTGGATCAT	45.0	50.0	58.3	58.3	477-526 bp	1.0	80.2
psbK-psbI_p5	GTTTGGCAAGCTGCTGTAAG	GGATTACGCCCTGGATCATT	50.0	50.0	57.9	57.4	477-526 bp	1.0	79.8