Hotspot

ndhF-rpl32

SSC · 111929-112881 · 953 bp

Back to Chrysosplenium

Pi 0.0678
MarkerSeek score 77.90
Primer available yes
Label ndhF-rpl32

Feature Metadata

label_name
ndhF-rpl32
feature_type
igs
region
SSC
length_bp
953
variable_sites
291
pi
0.0678
unique_haplotype_count
31
primer_available
yes
alignment_reliability
0.92
markerseek_score
77.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
ndhF-rpl32_p1 AGCAACTGCACCGGAAAATA AGCGTGTCTACCGATTTCAC 45.0 50.0 58.1 58.0 1993-2505 bp 0.939394 88.7
ndhF-rpl32_p2 TAGCAACTGCACCGGAAAAT AGCGTGTCTACCGATTTCAC 45.0 50.0 58.1 58.0 1994-2506 bp 0.939394 88.7
ndhF-rpl32_p3 AGCAACTGCACCGGAAAATA CAGCGTGTCTACCGATTTCA 45.0 50.0 58.1 58.0 1994-2506 bp 0.939394 88.7
ndhF-rpl32_p4 TAGCAACTGCACCGGAAAAT CAGCGTGTCTACCGATTTCA 45.0 50.0 58.1 58.0 1995-2507 bp 0.939394 88.7
ndhF-rpl32_p5 ATTTAGCAACTGCACCGGAA AGCGTGTCTACCGATTTCAC 45.0 50.0 58.1 58.0 1997-2509 bp 0.848485 85.1