Hotspot

rpl20-rps12

LSC · 68640-69366 · 727 bp

Back to Chrysosplenium

Pi 0.0346

MarkerSeek score 80.00

Primer available yes

Label rpl20-rps12

Feature Metadata

label_name: rpl20-rps12
feature_type: igs
region: LSC
length_bp: 727
variable_sites: 126
pi: 0.0346
unique_haplotype_count: 31
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 80.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpl20-rps12_p1	GTCAGTCTTGAATGAGCCCC	CGCCGAGGAACATGTACTAG	55.0	55.0	58.3	57.9	829-894 bp	0.969697	87.8
rpl20-rps12_p2	GTCAGTCTTGAATGAGCCCC	GCCGAGGAACATGTACTAGG	55.0	55.0	58.3	57.5	828-893 bp	0.969697	84.7
rpl20-rps12_p3	CCCGAAAGCTTGATGCAAAT	CGCCGAGGAACATGTACTAG	45.0	55.0	57.3	57.9	810-875 bp	0.969697	84.5
rpl20-rps12_p4	AGTCAGTCTTGAATGAGCCC	CGCCGAGGAACATGTACTAG	50.0	55.0	57.2	57.9	830-895 bp	0.969697	83.6
rpl20-rps12_p5	CCCGAAAGCTTGATGCAAAT	GCCGAGGAACATGTACTAGG	45.0	55.0	57.3	57.5	809-874 bp	0.969697	81.4