Hotspot

ndhC-trnV-UAC

LSC · 50915-51625 · 711 bp

Back to Chrysosplenium

Pi 0.0554

MarkerSeek score 81.70

Primer available yes

Label ndhC-trnV-UAC

Feature Metadata

label_name: ndhC-trnV-UAC
feature_type: igs
region: LSC
length_bp: 711
variable_sites: 194
pi: 0.0554
unique_haplotype_count: 31
primer_available: yes
alignment_reliability: 0.95
markerseek_score: 81.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhC-trnV-UAC_p1	TCATATTCGTAAAGCAGAAACA	CCGAGAAAGTCTACGGTTCG	31.8	55.0	53.9	58.1	528-890 bp	0.969697	50.8
ndhC-trnV-UAC_p2	ATCATATTCGTAAAGCAGAAACA	CCGAGAAAGTCTACGGTTCG	30.4	55.0	54.5	58.1	529-891 bp	0.969697	50.8
ndhC-trnV-UAC_p3	TCATATTCGTAAAGCAGAAACAT	CCGAGAAAGTCTACGGTTCG	30.4	55.0	54.5	58.1	528-890 bp	0.969697	50.8
ndhC-trnV-UAC_p4	TCATATTCGTAAAGCAGAAACA	GTTCGAGTCCGTATAGCCCT	31.8	55.0	53.9	58.7	513-875 bp	0.969697	50.8
ndhC-trnV-UAC_p5	CATATTCGTAAAGCAGAAACA	CCGAGAAAGTCTACGGTTCG	33.3	55.0	52.3	58.1	527-889 bp	0.969697	50.8