Hotspot

psbZ-trnG-GCC

LSC · 36336-36911 · 576 bp

Back to Chrysosplenium

Pi 0.0853

MarkerSeek score 74.20

Primer available yes

Label psbZ-trnG-GCC

Feature Metadata

label_name: psbZ-trnG-GCC
feature_type: igs
region: LSC
length_bp: 576
variable_sites: 206
pi: 0.0853
unique_haplotype_count: 30
primer_available: yes
alignment_reliability: 0.86
markerseek_score: 74.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psbZ-trnG-GCC_p1	CAAGACCGGAGCTATCAACC	CGTTGTTTTTCGACCGGAAG	55.0	50.0	58.1	58.0	1512-1839 bp	1.0	91.4
psbZ-trnG-GCC_p2	TGAGTCACCGAGCAAAACAA	CGTTGTTTTTCGACCGGAAG	45.0	50.0	58.0	58.0	1590-1917 bp	0.757576	81.9
psbZ-trnG-GCC_p3	TTGAGTCACCGAGCAAAACA	CGTTGTTTTTCGACCGGAAG	45.0	50.0	58.0	58.0	1591-1918 bp	0.757576	81.9
psbZ-trnG-GCC_p4	TGAGTCACCGAGCAAAACAA	CTTGAGGTCACGGGTTCAAA	45.0	50.0	58.0	58.0	1273-1594 bp	0.757576	81.7
psbZ-trnG-GCC_p5	TTGAGTCACCGAGCAAAACA	CTTGAGGTCACGGGTTCAAA	45.0	50.0	58.0	58.0	1274-1595 bp	0.757576	81.7